Immune Profiling
Maxpar Direct Immune Profiling Assay and Maxpar Pathsetter software
Identify major circulating immune cell types in human whole blood or PBMC samples with the validated, dry-format Maxpar® Direct™ Immune Profiling Assay™.
This robust immunophenotyping assay provides clinicians with an easy-to-implement solution for routine and reproducible sample collection to capture the highly individual nature of immune response.
Now available in two sizes, a 25-test kit and 5-test kit format.
Easily add the validated assay into clinical research studies
Hear Allie Greenplate, PhD, describe the rapid, standardized approach at the Immune Health Project at Penn Medicine to collect detailed immune response data from patients that can then be translated into actionable information for discovery and clinical care teams.
Overview
Standardize immune monitoring studies
A simple single-tube workflow with automated analysis minimizes variability and ensures high reproducibility of multiparameter data.
Proven performance and reproducibility
Clinical and translational research demands reproducible and reliable immune profiling, especially when samples are processed and analyzed by different technicians or at different study sites1,2. The reproducibility of the Maxpar Direct Immune Profiling System has been demonstrated run-to-run and site-to-site.
Workflow
Learn how the single-tube assay speeds up sample collection to insights.
Ideal for multicenter or longitudinal studies
Stain, freeze, ship
Unlike fluorescent tags that are light-sensitive, metal tags can withstand harsh staining protocols and storage conditions, providing greater stability when freezing or shipping samples.
Standardized sample collection
Maintain sample integrity between collection and processing by stabilizing whole blood with preservation reagents, even at room temperature.
Data Analysis
Actionable insights with rapid data
Maxpar® Pathsetter™ software is a fully automated reporting and data analysis solution that automatically identifies 37 immune cell types in FCS files from samples processed with the Maxpar Direct Immune Profiling Assay.
Expanding and Customizing
Easily build on the Maxpar Direct™ backbone panel with individual antibodies or Expansion Panels, designed to enable deeper profiling of specific immune cell subsets that are relevant in immune-mediated disease, such as infectious disease, cancer, autoimmunity or allergy.
9 Expansion Panels
- Surface profiles
- Cell proliferation
- Cell exhaustion
- Apoptosis
- Metabolism
- Phosphoproteins
- Cytokine production
- Transcription factors
Application Note
Building a 47-marker flow cytometry panel to explore antigen-specific immune responses. Download application note
Scientific Poster
A high-parameter mass cytometry panel for the functional characterization of CAR T cells. Download poster
Products
Related products that expand the core, backbone assay.
Maxpar® Direct™ T Cell Activation Expansion Panel
The Maxpar® Direct™ T Cell Activation Expansion Panel enables deep phenotyping and functional characterization of T cell activation and exhaustion in conjunction with the Maxpar® Direct™ Immune Profiling Assay™. The panel includes intracellular cytokine markers, which may be used to study the phenotype of cells responding to a particular treatment or stimulant and can also be used to identify the frequency of antigen-specific T cell responses after natural infection or vaccination. Additional markers included support the measurement of cytotoxic potential in T and natural killer (NK) cells and identification of activated T helper cell populations.MarkerCloneChannelPrimary PurposeStainingCD69FN50113CdEarly activation markerSurfaceCD107aH4A3106CdCytotoxic degranulation markerSurfaceCTLA-414D3162DyT cell exhaustion markerIntracellularGranzyme BGB11198PtCytotoxicity markerIntracellularIFNγB27116CdAnti-viral and immunoregulatory cytokineIntracellularIL-2MQ1-17H12112CdPleiotropic cytokineIntracellularIL-4MP425D2142NdAnti-inflammatory Th2 cytokineIntracellularIL-10JES3-9D7165HoRegulatory cytokineIntracellularIL-17ABL168169TmPro-inflammatory Th17 cytokineIntracellularPerforinB-D48196PtCytotoxicity markerIntracellularTNFαMab11114CdPro-inflammatory cytokineIntracellular
Maxpar® Direct™ Myeloid and Lymphoid Activation Expansion Panel
The Maxpar® Direct™ Myeloid and Lymphoid Activation Expansion Panel enables phenotyping and intracellular cytokine profiling of myeloid and lymphoid cell subsets in conjunction with the Maxpar® Direct™ Immune Profiling Assay™. The panel supports the functional characterization of cell types by including markers of inflammatory and suppressed monocytes, B cell regulation and activation, and T cell activation and exhaustion.MarkerCloneChannelPrimary PurposeStainingCD33WM53169TmLineage marker for myeloid populationsSurfaceCD405C3142NdAPC costimulatory moleculeSurfaceCD69FN50113CdEarly activation markerSurfaceCD802D10.4162DyAPC costimulatory moleculeSurfaceGM-CSFBVD221C11159TbGrowth factor, pro-inflammatory cytokineIntracellularIL-1βCRM56209BiPro-inflammatory cytokineIntracellularIL-6MQ2-13A5106CdPleiotropic cytokineIntracellularIL-10JES3-9D7165HoRegulatory cytokineIntracellularPD-1EH12.2H7175LuImmune cell exhaustion markerSurfaceTNFαMab11114CdPro-inflammatory cytokineIntracellular
Maxpar® Direct™ NK Cell Expansion Panel 1
The Maxpar® Direct™ NK Cell Expansion Panel 1 enables deeper phenotyping of NK cells in conjunction with the Maxpar® Direct™ Immune Profiling Assay™. This panel includes NK regulation and activation and exhaustion markers, which enable deeper profiling of NK subsets.TargetCloneMetalCD181/CXCR18F1/CXCR1142NdCD337/NKp30Z25159TbCD335/NKp46BAB281162DyCD279/PD-1EH12.2H7165HoCD159a/NKG2AZ199169TmCD278/ICOSC398.4A175LuTIGITMBSA43209Bi
Maxpar® Direct™ Myeloid and B cell Expansion Panel 1
Maxpar® Direct™ Myeloid and B Cell Expansion Panel 1 enables deeper phenotyping of myeloid and B cell subsets in conjunction with the Maxpar® Direct™ Immune Profiling Assay™. This panel includes markers for monocytic myeloid-derived suppressor cells, B cell regulation and activation, and neutrophil activation and exhaustion.TargetCloneMetalCD181/CXCR18F1/CXCR1142NdCD22HIB22159TbCD80/B7.12D10.4162DyCD163GHI/61165HoCD33WM53169TmCD279/PD-1EH12.2H7175LuCD11b/Mac-1ICRF44209Bi
Maxpar® Direct™ T-cell Expansion Panel 2
Maxpar® Direct™ T Cell Expansion Panel 2 enables investigation of T cell migration, activation and exhaustion in conjunction with the Maxpar® Direct™ Immune Profiling Assay™. This panel also enables in-depth assessment of circulating T follicular helper cells and T stem cell memory subset.TargetCloneMetalCD11aHI111142NdCD366/Tim-3F38-2E2159TbCD95/FasDX2162DyCD279/PD-1EH12.2H7165HoCD278/ICOSC398.4A169TmCD184/CXCR412G5175LuTIGITMBSA43209Bi
Resources
Publications
User guides
Technical documents
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Unless explicitly and expressly stated otherwise, all products are provided for Research Use Only, not for use in diagnostic procedures. Find more information here.