b'Human Solid TumorIn this application, breast cancer and colon cancer tissue samples were stained witha 41-marker panel comprised of the high-plex Human Immuno-Oncology IMC Panel, View the 31 Antibodies and expanded targets of interest, and acquired with WSI modes. study detailsIdentifying regions of immune activity and infiltration in tumor tissue2 mm 200 m2 mm200 m2 mmMultiple types of lymphoid and myeloid immune cells were detected at the tumor margin (red inset). A tumor structure with low immune penetration was detected surrounding the necrotic tissue (cyan inset). Single-cell analysis quantitatively delineated cell phenotype of PD-L1-expressing tumor and immune cell populations. The detection of subpopulations of tumor and immune cells defines the highly heterogeneous nature of the breast cancer sample. Revealing complex compartmentalization and tissue heterogeneity2 mmStriking heterogeneity of breast cancer tissue can be detected with specialized tumor structures across the tissue. Lymphocyte localization is observed at the tumor margins (A, inset). The presence of specialized tumor cells expressing iNOS (B, inset) and tissue compartments containing interactive niches of cancer-associated fibroblasts (CAFs) and immune cells (C, inset) are detected. Pixel-clustering analysis highlighted several tumor areas such as immune-deficient, highly replicative, immune cell-adjacent and stromal cell-adjacent tumor regions.'