b'IntroductionIdentifying cellular- and spatial-level composition of the tumor microenvironment (TME) is vital for interpretation of disease origin, progression, prognosis and treatment options. Two new whole slide imaging (WSI) modes and an integrated automated slide loader have been developed for Imaging Mass Cytometry (IMC) that enable streamlined workflows using ultrafast Preview Mode and high-throughput Tissue Mode. Novel WSI modes with the Hyperion XTi Imaging System provide rapid and detailed assessmentof tissue structure and cell features. This lookbook showcases translational and clinical applicationsof multiplexed tissue analysis.KEY TAKEAWAYSNew imaging modes provide a novel high-throughput workflow formultiplexed imaging of tumor samples.Using a combination of imaging modes and ready-to-go high-plex panels on the same tissue section provides researchers with more flexibility to understand the extensive cellular heterogeneity of the TME.Rapid imaging modes demonstrate key biological insights captured inspatial context that are relevant for identifying prognostic and diagnostic biomarkers for targeted therapies.2 mmPreview Mode Cell Mode Tissue ModeNumber of markers: 42Number of markers: 42Number of markers: 42 Acquisition time: 20 minutes Acquisition time: 2 hours Acquisition time: 5 hours and 50 minutes Sample: colon cancer (25 mm x 15 mm) Sample: colon cancer (2 mm x 2 mm) Sample: breast cancer (24 mm x 16 mm)Resolution: 1 m Resolution: 5 mIMMUNO-ONCOLOGY'